Chemiluminescence Immunoassay (CLIA) provides a sensitive, high throughput, fully automated immunological technique, which has revolutionized Immunodiagnostics. It measures the intensity of light emitted when the complex of antigen in the patient's sample and the enzyme linked antibody react with ch..
When molecules in the mixture are very similar, direct quantification becomes difficult. HPLC is a form of column chromatography used frequently to separate, identify and quantify compounds. It consists of a stationary phase that absorbs the analytes and holds them for a particular time. All the..
ELISA is an immunological technique which is used to detect the presence of an antibody or an antigen in a sample. A known amount of antigen is coated on a solid phase like that in the well of a polystyrene microtitre plate and sample containing specific antibodies is added in the wells to allow ant..
Photometry is the science of measuring visible light and is based on a relationship between absorption of light and the properties of the material through which the light is traveling (Beer Lambert's law).
Nephelometry is a quantitative method, based on the principle that a dilute suspension of small particles will scatter light (usually a laser) passed through it rather than simply absorbing it. The amount of scatter is determined by collecting the light at an angle.
In clinical chemistry, estimation of serum electrolytes is also an essential, as their concentrations affect variety of biological functions. The simple instrumentation and ease of operation makes it again a desirable addition to the lab floor to complete the clinical biochemistry profile